The whipping gusts that disrupted athletes and spectators alike were just a prelude to the winds of change that roared through Rio de Janeiro on Sunday night: Serena and Venus Williams lost an Olympic doubles match for the first time.

Day two of the Rio Games proved quite the breeze for some athletes and much too windy for others. The gusts ripped apart a large decorative panel on the swimming venue and even shut down shopping at the megastore — essentially an enormous tent — inside the Olympic Park.

Then, the tempest: the Williams sisters were stunned in the opening round by the Czech Republic’s Lucie Safarova and Barbora Strycova 6-3, 6-4 after entering Sunday’s match with a 15-0 mark in the Olympics.

They had won the gold medal in women’s doubles every time they entered the event: in 2000, 2008 and 2012. The American duo was seeded No. 1 in Rio and coming off a 14th Grand Slam championship together at Wimbledon a month ago.

China won yet another medal in air rifle on a day nasty winds sent the clay targets in the trap event bobbing and bouncing through the air, forced delays on the tennis courts and whipped up treacherous waves in the Rodrigo de Freitas Lagoon.

Sunday’s rowing regatta was called off after a two-hour delay when the choppy seas didn’t let up. Race officials said winds gusting up to 34 mph (15 meters per second) pushed buoys into the lanes and capsized two boats during morning practice.